Loading... Please wait...
EUR
- Home
- Antibodies
- KO Validated Antibodies
KO Validated Antibodies
KO Validated Antibodies
Every antibody in this range is benchmarked against a true CRISPR/Cas9 knockout cell line before it ships — so a positive signal means the protein is really there, not an artefact of cross-reactivity.
![Anti-EGFR [KO Validated] product image, KO validated antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/77258/460808/anti-egfr-antibody-cab11351ko-validated__61478.1631912917.jpg)
![Anti-HDAC8 [KO Validated] product image, KO validated antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/84613/375119/anti-hdac8-antibody-cab5829ko-validated__14547.1631167361.jpg)
![Anti-Bcl10 [KO Validated] product image, KO validated antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/77156/467355/anti-bcl10-antibody-cab1106ko-validated__15192.1631933576.jpg)
How Do We Validate Our Knockout Antibodies?
A three-step process built around a real genetic knockout, not a synthetic blocking control.
Generate the knockout
We create CRISPR knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/Cas9.
Run a parallel Western blot
The CRISPR knockout cells are lysed alongside wild-type (WT) control cells and run side-by-side on a Western blot.
Confirm loss of signal
Blots are probed with our antibody. A clean loss of signal in the knockout lane, against a persistent WT band, confirms high specificity.
Popular Validated Antibody Targets
A cross-section of our most requested KO validated antibodies, spanning six research areas.
Bcl10 Antibody [KO Validated]
CAPG Antibody [KO Validated]
Cytokeratin 8 Antibody [KO Validated]
GIT1 Antibody [KO Validated]
EEF1B2 Antibody [KO Validated]
AK2 Antibody [KO Validated]
CDK8 Antibody [KO Validated]
EGFR Antibody [KO Validated]
NF2 Antibody [KO Validated]
Browse All 570+ KO Validated Antibodies
Filter by application or search by target name/gene symbol to find the right knockout-validated antibody for your assay.
4E BP1 [R06-8E6] Antibody [KO Validated]
[KD]Cleaved-Caspase 9 [R05-4I8] Antibody [KO Validated]
ACAT2/Acetyl-CoA acetyltransferase Antibody [KO Validated]
Acetyl-HMGB1-K29 Antibody [KO Validated]
active + pro Caspase-3 Antibody [KO Validated]
Aldolase [R06-6A3] Antibody [KO Validated]
Annexin 4 [R04-3H8] Antibody [KO Validated]
Annexin A5/Annexin V Antibody [KO Validated]
BAF57/SMARCE1 Antibody [KO Validated]
Bax [ARC5006-10] Antibody [KO Validated]
Bcl-XL [R02-2D3] Antibody [KO Validated]
Beclin 1 [R07-2K6] Antibody [KO Validated]
beta 2 Microglobulin Antibody [KO Validated]
beta Catenin Antibody [KO Validated]
beta-Catenin Antibody [KO Validated]
betaIII-Tubulin Antibody [KO Validated]
BRG1/SMARCA4 Antibody [KO Validated]
Caldesmon/CDM [R05-3D2] Antibody [KO Validated]
cAMP Protein Kinase Catalytic Subunit [R07-8H3] Antibody [KO Validated]
Caspase 9 [R09-4C4] Antibody [KO Validated]
Catalase [R03-8E9] Antibody [KO Validated]
CD147 [R04-5B3] Antibody [KO Validated]
CDC42 [R01-1C7] Antibody [KO Validated]
CDK2 [R04-1D5] Antibody [KO Validated]
Cofilin 1 [R06-2C0] Antibody [KO Validated]
CRABP2 [R01-4C4] Antibody [KO Validated]
Creatine Kinase B type [R03-4C1] Antibody [KO Validated]
Creatine Kinase B type [R03-5I4] Antibody [KO Validated]
Cyclin B1 [R08-9B7] Antibody [KO Validated]
Cytochrome C Antibody [KO Validated]
Cytokeratin 8 Antibody [KO Validated]
Cytokeratin 8 Antibody [KO Validated]
DARPP32 [R07-4D3] Antibody [KO Validated]
DCAMKL1 [R05-8D6] Antibody [KO Validated]
DKC1 [R02-2D8] Antibody [KO Validated]
DNA Polymerase beta Antibody [KO Validated]
EGFR [R01-2D3] Antibody [KO Validated]
EGFR [R03-6B1] Antibody [KO Validated]
eIF3B [R09-4I5] Antibody [KO Validated]
Eph receptor B4/HTK [R04-3B0] Antibody [KO Validated]
Fascin/FSCN1 Antibody [KO Validated]
Fatty Acid Synthase (FASN) Antibody [KO Validated]
FERMT2 Antibody [KO Validated]
Flotillin 1 [R08-5E9] Antibody [KO Validated]
G3BP [R06-5D2] Antibody [KO Validated]
Galectin 3/LGALS3 Antibody [KO Validated]
GCLC [R03-9D9] Antibody [KO Validated]
Glucocorticoid Receptor Antibody [KO Validated]
Glutamine Synthetase (GLUL) Antibody [KO Validated]
Glutathione Peroxidase 1 [R06-0H1] Antibody [KO Validated]
Glutathione Synthetase (GSS) Antibody [KO Validated]
GSK3 alpha/beta [R08-8G7] Antibody [KO Validated]
HIF1AN/FIH1 Antibody [KO Validated]
Histone H2B Antibody [KO Validated]
HP1 gamma/CBX3 Antibody [KO Validated]
HuR/ELAVL1 Antibody [KO Validated]
Insulin degrading enzyme Antibody [KO Validated]
Insulin-degrading enzyme (IDE) Antibody [KO Validated]
Integrin alpha 2 (ITGA2/CD49b) Antibody [KO Validated]
Integrin alpha 5 [R02-3G4] Antibody [KO Validated]
Integrin alpha V [R02-6G3] Antibody [KO Validated]
Integrin alpha V [R03-6E3] Antibody [KO Validated]
Lamin A/C [ARC5001-08] Antibody [KO Validated]
Leukotriene A4 Hydrolase Antibody [KO Validated]
Lipoamide Dehydrogenase Antibody [KO Validated]
LRP1 [R09-4E8] Antibody [KO Validated]
MAD2L2 [R08-7E2] Antibody [KO Validated]
MBD2 [R05-4G5] Antibody [KO Validated]
Mitofusin 1 [R05-3A8] Antibody [KO Validated]
N-Cadherin Antibody [KO Validated]
NAK/TBK1 [R01-2C4] Antibody [KO Validated]
NAK/TBK1 [R05-2H7] Antibody [KO Validated]
NF-kB p65/RelA Antibody [KO Validated]
nmt55 / p54nrb Antibody [KO Validated]
P70 S6 Kinase beta/SRK [R04-9C1] Antibody [KO Validated]
PDGFR beta [R02-5B2] Antibody [KO Validated]
Peroxiredoxin 4 (PRDX4) Antibody [KO Validated]
Phospho-4E BP1 (Thr46) [R03-5C7] Antibody [KO Validated]
Phospho-Cyclin B1 (Ser126) [R05-3B3] Antibody [KO Validated]
Phospho-HSF1 (Ser303/Ser307) [R01-7G4] Antibody [KO Validated]
Phospho-HSF1 (Ser326) [R08-4I1] Antibody [KO Validated]
Phospho-NDRG1 (Ser330) [R05-1D2] Antibody [KO Validated]
Phospho-PKC alpha (Ser657) [R05-8I3] Antibody [KO Validated]
Phospho-PKC alpha (Thr638) [R09-7C7] Antibody [KO Validated]
Phospho-PKC gamma (Thr514) [R02-4F3] Antibody [KO Validated]
Phospho-Presenilin 1 (Ser310) [R07-6E3] Antibody [KO Validated]
Phospho-STAT3 (Tyr705) [R06-6L7] Antibody [KO Validated]
PKA C-alpha (PRKACA) Antibody [KO Validated]
PKA RII alpha (PRKAR2A) Antibody [KO Validated]
PKC alpha [R05-4E5] Antibody [KO Validated]
PKC delta [R09-8H6] Antibody [KO Validated]
Placental Alkaline Phosphatase [R01-8I3] Antibody [KO Validated]
Presenilin 1 [R05-4D4] Antibody [KO Validated]
PSMB10/MECL1 [R08-6I5] Antibody [KO Validated]
Pyruvate Dehydrogenase E2 [R01-6I2] Antibody [KO Validated]
Rab5 [R01-6G5] Antibody [KO Validated]
Rel B [R07-9J3] Antibody [KO Validated]
SMARCB1/SNF5 Antibody [KO Validated]
SQSTM1/p62 Antibody [KO Validated]
SQSTM1/p62 Antibody [KO Validated]
STAT3 [R08-6C2] Antibody [KO Validated]
Staufen [R03-3G2] Antibody [KO Validated]
TAF15 [R04-8A3] Antibody [KO Validated]
Thimet Oligopeptidase Antibody [KO Validated]
Thyroid Transcription Factor 1 [R02-6A5] Antibody [KO Validated]
TTC11/FIS1 [ARC5010-03] Antibody [KO Validated]
What Is KO Validation?
KO validation is a means by which antibody specificity is confirmed by testing antibodies in a knockout (KO) cell line. Using KO cell lines — cells with no expression of the target protein — is the new gold standard for validating antibody specificity.
If a signal is detected from a KO cell line, the antibody is not highly specific; this is due to the antibody binding to a similar protein within the sample. Antibodies with high specificity, on the other hand, will not produce any signal in the knockout. KO validated antibodies must be shown to be specific, selective and reproducible.
Why Is Validation Important?
Antibodies are commonly used research tools, applied routinely in experiments such as Western blot, ELISA, immunostaining (IHC/ICC), immunofluorescence and flow cytometry. For the findings of these techniques to be reliable, they must have an internally high standard of accuracy and precision.
This is why there is a need for highly specific antibodies that can ensure reproducibility of results — and why every antibody in this range is put through the KO validation process described above before it's listed for sale.
Related Antibodies
Looking for a particular antibody? Use our Live Chat to get in touch and one of our specialists will be happy to help.
Secondary Antibodies
HRP, AP and fluorophore-conjugated secondaries to pair with any of our KO validated primary antibodies.
Browse Secondary AntibodiesFC Antibodies
Flow cytometry antibodies for immunophenotyping and functional studies across human, mouse and rat panels.
Browse FC AntibodiesChIP Antibodies
Chromatin immunoprecipitation-grade antibodies for studying protein–DNA interactions and epigenetic marks.
Browse ChIP AntibodiesWhy Choose Assay Genie
CRISPR/Cas9 Verified
Every antibody benchmarked against a true genetic knockout, not a blocking peptide.
PhD-Level Technical Support
Free help choosing the right clone, dilution or application for your model.
Reproducible By Design
Built to eliminate false-positive bands and non-specific staining from your data.
Frequently Asked Questions
What does “KO Validated” mean on an antibody datasheet?
It means the antibody has been tested side-by-side on a CRISPR/Cas9 knockout (KO) cell line and its wild-type (WT) counterpart by Western blot. If the signal disappears in the KO sample while the WT band remains, the antibody is confirmed to bind specifically to the intended target rather than a similar off-target protein.
How does Assay Genie perform KO validation?
We generate CRISPR knockout cell lines using sgRNA/Cas9 to introduce frame-shift mutations into the target gene, lyse both the KO and wild-type cells, and run parallel Western blots probed with the antibody. A clean loss of signal in the KO lane, against a persistent band in the WT lane, is the pass criterion before an antibody is listed as KO Validated.
Why is KO validation considered the gold standard for antibody specificity?
Peptide-blocking and overexpression assays can still leave cross-reactivity with related family members undetected. A true genetic knockout removes the target protein entirely, so any signal that remains can only be non-specific binding — making it the most rigorous specificity test currently available for research antibodies.
Which applications are KO validated antibodies suitable for?
Every antibody in this range is validated by Western blot (WB). Many are additionally tested and recommended for ELISA, immunohistochemistry (IHC), immunofluorescence/ICC, immunoprecipitation (IP), flow cytometry (FC) or ChIP — check the Application field on each individual product page for that specific antibody.
Can I use a KO validated antibody in a species that wasn’t part of the knockout study?
Reactivity is listed on each product page (for example Human, Mouse, Rat). Knockout validation is typically performed in one species/cell line, but sequence homology across the listed reactive species means the antibody is generally reliable in those species too — contact our technical team if you'd like help confirming cross-reactivity for your specific model.
What if my target isn’t among the 570+ KO validated antibodies listed here?
Get in touch via Live Chat or our contact form — we're continually expanding this catalogue and can advise on the closest validated alternative, or discuss a custom antibody development project for your target.
Do KO validated antibodies cost more than standard antibodies?
No — pricing follows our standard catalogue tiers by format and pack size. KO validation is part of our routine quality-control process for this range, not a premium add-on.
Ready For Antibodies You Can Trust?
Browse the full KO validated range, or talk to our team about a target that isn't listed yet.