Human GR alpha/Glucocorticoid Receptor Alpha ELISA Kit
The Human GR (Glucocorticoid Receptor) Alpha ELISA Kit is a powerful tool for quantifying levels of GR Alpha in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it a valuable asset for a variety of research purposes. The glucocorticoid receptor alpha is a key player in regulating gene expression in response to glucocorticoid hormones, which play a crucial role in various physiological processes such as immune response, metabolism, and stress regulation. Dysregulation of GR Alpha signaling has been implicated in a range of diseases including inflammatory disorders, autoimmune conditions, and mental health disorders, highlighting its importance as a potential therapeutic target.
By accurately measuring GR Alpha levels, researchers can gain valuable insights into the mechanisms underlying these diseases and identify potential therapeutic strategies. The Human GR Alpha ELISA Kit provides a reliable and efficient solution for studying the role of GR Alpha in health and disease, offering researchers a powerful tool for advancing their scientific understanding.
Product Name:
Human GR alpha/Glucocorticoid Receptor Alpha ELISA Kit
SKU:
HUFI02526
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
91-105%
83-99%
81-99%
EDTA Plasma (n = 5)
86-104%
85-100%
81-98%
Heparin Plasma (n = 5)
92-104%
82-96%
81-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-97
93
EDTA Plasma (n = 5)
91-103
96
Heparin Plasma (n = 5)
89-101
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
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