Human CRH (Corticotropin Releasing Hormone) ELISA Kit
The Human CRH (Corticotropin-Releasing Hormone) ELISA Kit is specifically designed for the precise measurement of CRH levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CRH is a vital hormone that plays a key role in the body's stress response system, regulating various physiological processes including metabolism, immune response, and behavior. Abnormal CRH levels have been linked to conditions such as anxiety disorders, depression, and chronic stress-related diseases, making it a valuable biomarker for studying these disorders and exploring potential treatment options.Overall, the Human CRH ELISA Kit provides researchers with a reliable tool for investigating the role of CRH in human health and disease, paving the way for new discoveries in the field of neuroendocrinology.
Product Name:
Human CRH (Corticotropin Releasing Hormone) ELISA Kit
SKU:
HUES02230
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
2.35 pg/mL
Detection range:
3.91-250 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
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