The Epinephrine ELISA Kit offered by Assay Genie is a cutting-edge tool for the precise measurement of epinephrine levels in various biological samples including serum, plasma, and tissue culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications. Epinephrine, also known as adrenaline, is a vital hormone and neurotransmitter that plays a crucial role in the body's response to stress and danger. It acts as a powerful stimulant, increasing heart rate, dilating airways, and mobilizing energy reserves to prepare the body for action.
Dysregulation of epinephrine levels has been linked to various medical conditions such as heart disease, asthma, and anxiety disorders. By accurately measuring epinephrine levels, researchers can gain valuable insights into the physiological and pathological processes involving this key molecule. This ELISA kit provides a reliable and efficient method for studying the role of epinephrine in health and disease, enabling advancements in biomedical research and drug development. Trust Assay Genie's Epinephrine ELISA Kit for dependable and insightful results in your studies.
Product Name:
Epinephrine ELISA Kit
SKU:
UNFI0039
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
85-104%
92-104%
98-105%
EDTA Plasma (n = 10)
83-98%
89-101%
84-100%
Heparin Plasma (n = 10)
82-100%
81-96%
88-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
86-102
91
EDTA Plasma (n = 10)
85-101
94
Heparin Plasma (n = 10)
87-105
97
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
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