Each category is a distinct type of post-translational modification — acetylation or methylation — that a residue on a histone or other protein can carry. The antibody you need depends on which specific mark (e.g. H3K9ac vs H3K9me3) you're trying to detect, since these antibodies are raised to recognise one modified state at one residue.

It varies by antibody: most are validated for Western blot (WB), with many also validated for ChIP, ChIP-seq, immunohistochemistry (IHC), immunofluorescence (IF/ICC), immunoprecipitation (IP), ELISA or dot blot (DB). Check the Applications listed for each specific antibody before ordering.

These antibodies are raised against synthetic peptides carrying the specific modification at the target residue, then affinity-purified against that immunogen. Datasheets include recommended dilutions and, where available, validation data such as western blots against modified versus unmodified peptide or knockdown controls.

Many of the histone methylation antibodies on this page are validated for ChIP and ChIP-seq — look for "ChIP" or "ChIP-seq" in the Applications tags. If you don't see it listed for your target, contact our technical support team to check availability.

Reactivity is listed on each antibody's individual product page. Because histone sequences are highly conserved, many of these antibodies show broad reactivity across human, mouse and rat samples, though this should always be confirmed per product.

Get in touch with our PhD-level technical support team or ask about our custom antibody development service — we can advise on the closest validated alternative or develop a bespoke antibody for your specific modification site.