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- Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin (IVMB0177)
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Product Description
Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the Ly-6C protein, a marker crucial for identifying subsets of monocytes, macrophages, and T-cells, making it an invaluable tool for research in immunology and related fields. With a rat IgG2b isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. Ly-6C is a protein expressed on the surface of immune cells, including certain monocytes, macrophages, and subsets of T-cells. It plays a significant role in the immune system by helping to distinguish between different subpopulations of immune cells and facilitating their functions in immune responses and inflammation.
| Product Name: | Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0177 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | HK1.4 |
| Protein: | Ly-6C |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Lymphocyte antigen 6 complex, locus C |
| Isotype: | Rat IgG2c |
| Reactivity: | Mouse |
| Immunogen: | L3 cloned CTL cells |
| Applications: | CyTOF®, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, CyTOF®, IHC FF, IP, WB |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Rat Anti-Mouse Ly-6C (Clone HK1.4) recognizes an epitope on Mouse Ly-6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5 |
| Immunogen: | L3 cloned CTL cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
| Datasheet: | View |
| Protein: | Ly-6C |
| Research Area: | Immunology |
