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- Anti-Mouse CD3epsilon [500A2] In Vivo Antibody - Low Endotoxin (IVMB0150)
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Product Description
Anti-Mouse CD3ε [500A2] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD3 [500A2] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD3 protein, which is essential for T-cell receptor signaling, making it ideal for research in immunology and related fields. With a hamster IgG isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for applications such as ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD3 is a multi-subunit protein complex found on the surface of T-cells. It plays a significant role in the immune system by participating in the activation and differentiation of T-cells through interaction with the T-cell receptor (TCR). This interaction is crucial for antigen recognition and the subsequent immune response.
| Product Name: | Anti-Mouse CD3epsilon [500A2] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0150 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 500A2 |
| Protein: | CD3-epsilon |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CD3-epsilon, T3, CD3 |
| Isotype: | Syrian Hamster IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse T-cell receptor |
| Applications: | B, FC, IHC FF |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, FC, IHC FF |
| Recommended Usage: | FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Syrian Hamster |
| Specificity: | Clone 500A2 recognizes an epitope on mouse CD3-epsilon. |
| Antigen Distribution: | CD3-epsilon is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. |
| Immunogen: | Mouse T-cell receptor |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
| Datasheet: | View |
| Protein: | CD3-epsilon |
| Function: | TCR signal transduction, T cell activation and antigen recognition. |
| Ligand/Receptor: | Peptide antigen/MHC-complex. |
| Research Area: | Cell Biology, Immunology, Signal Transduction |
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