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- Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin (IVMB0148)
Product Description
Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications. This antibody targets the CD25 protein, an essential component in immune response, making it an excellent tool for research in immunology and related fields. With a rat IgG1 isotype, it guarantees high purity and low endotoxin levels (<1.0 EU/mg), ensuring optimal performance in ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Elevate your research with this dependable and versatile antibody.
CD25, also known as the interleukin-2 receptor alpha chain, is prominently expressed on the surface of activated T-cells, regulatory T-cells, and some B cells. It plays a critical role in the immune system by modulating the activity of T-cells and regulating immune tolerance. This makes the Anti-Mouse CD25 In Vivo Antibody an invaluable tool for studying T-cell regulation, immune response, and therapeutic interventions. Enhance your immunological research with the Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin from Assay Genie, ensuring accurate and reliable results.
| Product Name: | Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0148 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | PC61 |
| Protein: | CD25 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IL-2Rα, Ly-43, p55, Tac |
| Isotype: | Rat IgG1 Lambda |
| Reactivity: | Mouse |
| Immunogen: | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Applications: | B, Depletion, FA, FC, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, Depletion, FA, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone PC61 recognizes an epitope on mouse CD25. |
| Antigen Distribution: | CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. |
| Immunogen: | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
| Datasheet: | View |
| Protein: | CD25 |
| Function: | Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132) |
| Ligand/Receptor: | IL-2 |
| Research Area: | Immunology |
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| Silvia Calvo et al. | Correlation between systemic allergen desensitisation and long-term asthma protection in mice following intravenous administration of the live tuberculosis vaccine MTBVAC | eBioMedicine 2024 | PubMed ID: 39173529 |
